#VECTOR TD UNBLOCKED AT SCHOOL PATCH#

Intratumoral injection of HSV/EBV amplicon vector encoding the mutant sodium channel and systemic administration of doxycycline led to regression of subcutaneous tumors in nude mice as assessed by in vivo bioluminescence imaging. In addition to cells expressing the mutant channel, adjacent, noninfected cells connected by gap junctions also died. Within hours, tumor cells swelled and died.

#VECTOR TD UNBLOCKED AT SCHOOL PATCH#

Upon infection and doxycycline induction of mutant channel expression in tumor cells, the open channel led to amiloride-sensitive sodium influx as assessed by patch clamp recording and sodium imaging in culture. This channel was delivered to tumor cells using a herpes simplex virus-1/Epstein–Barr virus (HSV/EBV) hybrid amplicon vector in which gene expression was controlled by a tetracycline regulatory system (tet-on) with silencer elements.

In this study, a novel tumor therapy was achieved by the delivery of a mammalian brain sodium channel, ASIC2a, carrying a mutation that renders it constitutively open. Viral vectors have been used to deliver a wide range of therapeutic genes to tumors.

Vector td unblocked at school